Types of Tissue and Muscle

Types of Tissue and Muscle Muscle Muscle is one of the four fundamental types of tissue present in animals. It is a soft tissue primarily responsible to produce movement of a body part. Its cells convert the chemical energy of ATP into the mechanical energy of motion and exert a useful pull on another tissue. More specifically, muscle contraction serves the following overlapping functions: movement and contractile move of body contents in the course of respiration, circulation, digestion, defecation, urination, and childbirth. Stability by resisting the pull of gravity. Communication. As well as the control of body openings and passages. And finally, producing around 85% of our body heat, which is vital for the metabolism (Carey Carpenter, 2007). Classification There are three histological types of muscle—cardiac, smooth and skeletal—which differ in appearance, physiology, and function. The cardiac muscle is essentially limited to the heart, though it extends slightly into the nearby blood vessels. It is involuntary and striated because of the regular arrangement of their actin and myosin filaments. Its cells are much shorter, so they are commonly called myocytes, and are mononuclated (Saladin, 2003). The myocytes assemble branches of adjoining cells and form a network by attaching to each other in attachment points called intercalated discs that provide strong mechanical adhesions between adjacent cells. Smooth muscle lacks striations and is involuntary. Smooth muscle cells are usually long and spindle-shaped, and each fusiform cell has a single and centered nucleus (Purves et al., 2004). Small amounts of smooth muscle are found in the iris of the eye and in the skin, but most of it, called visceral muscle, forms layers in the walls of the digestive, respiratory, and urinary tracts, blood vessels, the uterus, and other viscersa (Alberts et al., 2008). The skeletal muscle type is of special interest of this thesis and will be further studied in the following sections. Skeletal muscle Skeletal muscles are, as the name implies, are bound to the skeleton by means of tendons, which means that they are volitional. It is composed of both muscular tissue and connective. A skeletal muscle cell (muscle fiber) is about 10 to 100m in diameter and 30 cm long. It is surrounded by a sparse layer of areolar connective tissue called the endomysium, which allows room for blood capillaries and nerve fibers to reach each muscle fiber. Muscle fibers are grouped in bundles called fascicles, which are visible to the naked eye as parallel strands. Each fascicle is separated from neighboring ones by a connective tissue sheath called the perimysium, usually somewhat thicker than the endomysium. The muscle as a whole is surrounded by still another connective tissue layer, the epimysium. The epimysium grades imperceptibly into connective tissue sheets called fasciae, deep fasciae between adjacent muscles and a superficial fascia (hypodermis) between the muscles and skin. It is described as striated and voluntary (Saladin, 2003). Structure The skeletal muscle tissue consists of long and cylindrical cells called muscle fibers, which are actually huge single cells that form during development by the fusion of many separate cells, called myoblasts. Each cell contains multiple nuclei adjacent to the plasma membrane, and they are about 10 to 100m in diameter and 30 cm long. The bulk of the cytoplasm inside is made up of myofibrils, which is the name given to the basic contractile elements of the muscle cell. Within each myofibril are thin actin filaments and thick specific muscle isoforms of myosin II filaments (Alberts et al., 2008). Myosin filaments are bundles of molecules with globular heads and polypeptide tails. Actin filaments consist of two chains of actin monomers twisted together. They are wrapped by chains of the polypeptide tropomyosin and studded at intervals with another protein, troponin. In most regions of the myofibril, each thick myosin filament is surrounded by six thin actin filaments, and conversely, each thin actin filament sits within a triangle of three thick myosin filaments. The myofibril consists of repeating units, called sarcomeres, which are the units of contraction, built up of approximately 2.2 microns in length. Each sarcomere is made of overlapping filaments of actin and myosin, which create a distinct band pattern. As the muscle contracts, the sarcomeres shorten, and the appearance of the band pattern changes. Each sarcomere is bounded by Z-lines, which are structures that anchor the thin actin filaments. Centered in the sarcomere is the A-band, which contains all the myosin filaments. The H-zone and the I-band, are regions where actin and myosin filaments do not overlap in the relaxed muscle. The dark stripe within the H-zone is called the M-band; it contains proteins that help hold the myosin filaments in their regular arrangement. The bundles of myosin filaments are held in a centered position within the sarcomere by a protein called titin. Cardiac muscle and smooth muscle also contain sarcomeres, although the organization is not as regular as that in skeletal muscle (Alberts et al., 2008; Purves et al., 2004.). The plasma membrane, called the sarcolemma has tunnel-like infoldings called transverse (T) tubules that penetrate through the fiber and emerge on the other side. The function of a T tubule is to carry an electrical current from the surface of the cell to the interior when the cell is stimulated. Most other organelles of the cell, such as mitochondria and smooth endoplasmic reticulum (ER), are located between adjacent myofibrils. The sarcoplasm also contains an abundance of glycogen, which provides stored energy for the muscle to use during exercise, and a red pigment called myoglobin, which binds oxygen until it is needed for muscular activity. The smooth ER of a muscle fiber is called sarcoplasmic reticulum (SR). It forms a network around each myofibril, and alongside the T tubules it exhibits dilated sacs called terminal cisternae. The SR is a reservoir for calcium ions; it has gated channels in its membrane that can release a flood of calcium into the cytosol, where the calcium a ctivates the muscle contraction process (Saladin, 2003). Muscle contraction For contraction to occur, an action potentials spreads from the end plate and when it reaches the T tubules, it continues down them into the sarcoplasm. Action potentials open voltage-regulated ion gates in the T tubules. These are physically linked to calcium channels in the terminal cisternae of the sarcoplasmic reticulum (SR), so gates in the SR open as well and calcium ions diffuse out of the SR, down their concentration gradient and into the cytosol. The calcium ions bind to the troponin of the thin filaments. The troponin-tropomyosin complex changes shape and shifts to a new position. This exposes the active sites on the actin filaments and makes them available for binding to myosin heads; the myosin heads must have an ATP molecule bound to it to initiate the contraction process. Myosin ATPase, an enzyme in the head, hydrolyzes this ATP. The energy released by this process activates the head, which â€Å"cocks† into an extended, high-energy position. The head temporarily keeps the ADP and phosphate group bound to it. The cocked myosin binds to an active site on the thin filament. Myosin releases the ADP and phosphate and flexes into a bent, low-energy position, tugging the thin filament along with it. This is called the power stroke. The head remains bound to actin until it binds a new ATP. Upon binding more ATP, myosin releases the actin. It is now prepared to repeat the whole process—it will hydrolyze the ATP, recock (the recovery stroke), attach to a new active site farther down the  thin filament, and produce another power stroke (Saladin, 2003). Classes of muscle fibers Not all muscle fibers are metabolically alike or adapted to perform the same task. Some respond slowly but are relatively resistant to fatigue, while others respond more quickly but also fatigue quickly. Indeed, skeletal muscles can be divided into fast and slow twitch fibers and its myosin heavy chain (MHC) isoform expression. Type I (MHC-I): Also called slow oxidative (SO) or slow-twitch. These fibers have relatively abundant mitochondria, myoglobin, and blood capillaries, and therefore a relatively deep red color. They are well adapted to aerobic respiration, which does not generate lactic acid. Thus, these fibers do not fatigue easily. However, in response to a single stimulus, they exhibit a relatively long twitch, lasting about 100 milliseconds (msec). Type II (MHC-II): Also called fast glycolytic (FG) or fast-twitch. They are well adapted for quick responses but not for fatigue resistance. They are rich in enzymes of the phosphagen and glycogen–lactic acid systems. Their sarcoplasmic reticulum releases and reabsorbs Ca2 quickly, which partially accounts for their quick, forceful contractions. They are relatively pale (white fibers). These fibers produce twitches as short as 7.5 msec, but because of the lactic acid they generate, they fatigue more easily than SO fibers. Some authorities recognize two subtypes of FG fibers called types MHC-IIA and MHC-IIB. Type IIB is the common type just described, while IIA, or intermediate fibers, combine fast-twitch responses with aerobic fatigue-resistant metabolism. Type IIA fibers, however, are relatively rare except in some endurance-trained athletes (Saladin, 2003). Notably, human skeletal muscle does not contain MHCIIb (Spangenburg and Booth, 2003; Schiaffino and Reggiani, 1994; Smerdu et al., 1994). In addition, ‘‘hybrid’’ fibers containing two MHC isoforms (i.e., type I/IIA, IIAX, IIXB) can also be present in muscle (Schiaffino and Reggiani, 1994; Staron and Pette, 1993). The fiber types can be differentiated histologically by using stains for certain mitochondrial enzymes and other cellular components, like using immunohistochemical procedures with antibodies against the specific MHC isoforms (Schiaffino et al., 1989; Lucas et al., 2000). All muscle fibers of one motor unit belong to the same physiological type. Nearly all muscles are composed of both SO and FG fibers, but the proportions of these fiber types differ from one muscle to another. Muscle myogenesis Vertebrate skeletal myogenesis proceeds through three stages: determination of the muscle progenitor cells, called myoblasts; proliferation and in some cases migration of myoblasts; and their terminal differentiation into mature muscle by fusing to form multinucleated myotubes (Buckingham et al., 2003; Shi and Garry, 2006). Muscle development Muscle tissuesare derived from themesodermallayer of embryonicgerm cellsin a process known asmyogenesis. All muscles are derived fromparaxial mesoderm [8].The paraxial mesoderm is divided along the embryos length intosomites, corresponding to thesegmentationof the body.Muscle cells come from two cell lineages in the myotome somite, the epimere and hypomere, which formepaxialandhypaxialmuscles, respectively. Most muscles are hypaxial. During development,myoblasts either remain in the somite to form muscles associated with the vertebral column or migrate out into the body to form all other muscles. Myoblast migration is preceded by the formation ofconnective tissueframeworks, usually formed from the somaticlateral plate mesoderm.Myoblasts follow chemical signals to the appropriate locations, where they fuse into elongate skeletal muscle cells (Sweeney, 1997). Muscle differentiation Muscle fibersform from the fusion ofmyoblastsinto multi-nucleated fibers calledmyotubes. In the early development of an embryothese myoblasts will proliferate if enoughfibroblast growth factor(FGF) is present, without differentiating. When these factors are depleted, the myoblasts cease division and secretefibronectinonto theirextracellular matrix and bind to it through _5ÃŽ ²1 integrin, their major fibronectin receptor (Menko and Boettiger 1987; Boettiger et al. 1995). The second stage involves the alignment of the myoblasts together into chains and subsequently into myotubes. This step is mediated by cell membrane glycoproteins, including several cadherins and CAMs (Knudsen 1985: Knudsen et al. 1990). Recognition and alignment between cells takes place only if the two cells are myoblasts. However, identity of the species is not critical (Yaffe and Feldman, 1965). The third stage is the actual cell fusion itself. In this stage,calciumions are critical for development (Shainberg et al. 1969; David et al. 1981). Fusion is mediated by a set of metalloproteinasescalledmeltrins (e.g., c-Met).Myocyte enhancer factors(MEFs) promote myogenesis.Serum response factor(SRF) plays a central role during myogenesis, being required for the expression of striated alpha-actin genes (Wei et al., 1998).Expression of skeletalalpha-actinis also regulated by theandrogen receptor; steroids can thereby regulate myogenesis (Vlahopoulos et al., 2005). The specific extracellular signals that induce determination of each group of myoblasts are expressed only transiently. These signals trigger production of intracellular factors that maintain the myogenic program after the inducing signals are gone. We discuss the identification and functions of these myogenic proteins, and their interactions, in the next several sections. Muscle-specific transcription factors Pax family Satellite cells and proliferating myoblasts is characterized by the expression of Pax-genes, more specifically Pax7 and Pax3, which are transcription factors that regulate proliferation. Back to the developmental stage, in the lateral portion of the somite, which forms the hypaxial muscles, factors from the surrounding environment induce the Pax3 transcription factor. In the absence of other inhibitory transcription, Pax3 then activates the genes encoding two muscle-specific transcription factors, Myf5 and MyoD. In the medial region of the somite, which forms the epaxial muscles, MyoD is induced through a slightly different pathway[1]. Pax7 residing satellite cells proliferating stage and Pax7 knockout mice completely lack satellite cells (Seale et al., 2000). Cells expressing Pax7 is believed to be important for the regeneration of myoblasts. Also the expression of Pax3 has negative effect on the differentiation and it has been shown that this transcription factor is down-regulated in muscle by ubiquitination and proteasomal degradation before differentiation can proceed (Boutet et al., 2007). Pax genes have been shown to regulate the proliferation and survival even of certain cancers such as melanoma (Muratovska et al., 2003). Pax transcription factors are downregulated upon further differentiation and other proteins predominate. Meanwhile Pax3 _____, Pax7 appears only in muscle stem cells (___). MyoD family Muscle cells come from two cell lineages in the somite. In both instances, paracrine factors instruct the myotome cells to become muscles by inducing them to synthesize the MyoD proteins (Maroto et al. 1997; Tajbakhsh et al. 1997). Or also called the myogenic bHLH (basic helix-loop-helix) proteins. The proteins of this family all bind to similar sites on the DNA and activate muscle-specific genes (e.g. the muscle-specific creatine phosphokinase gene by binding to the DNA immediately upstream from it, or the chicken muscle acetylcholine receptor) (Lassar et al. 1989; Piette et al. 1990). MyoD and Myf5 belong to this family and are particularly important for muscle differentiation [2]. Their important role during differentiation is supported by the MyoD-/-/Myf-5-/- mice lacking fully developed skeletal muscle (Rudnicki et al., 1993). Myf-5 promotes myoblasts’ proliferation and is required for the cells to initiate differentiation (Ustanina et al., 2007). Absence of MyoD inhibits differentiation in cell culture and the protein is therefore considered to be a positive regulator of the process (Sabourin et al., 1999). While Pax3 is found in several other cell types, the myogenic bHLH proteins are specific for muscle cells. Any cell making a myogenic bHLH transcription factor such as MyoD or Myf5 is committed to becoming a muscle cell. Myogenic regulatory factors (proper name?) Later than the MyoD proteins expression during differentiation, the myogenin and the myogenic regulatory factor 4 (MRF4) are present. Mice with deleted myogenin in developing myoblasts can start the differentiation process but they cannot move and die soon after birth (Hasty et al., 1993). Among other things, this suggests that myogenin is required at a later stage of the process. Similarly, MRF4 is important for the growth of muscle tissue (Rhodes and Konieczny, 1989). It is noted that the MEF2 family of transcription factors also regulate differentiation (Olson et al., 1995), but their functions are not described in this context. In summary, Pax transcription factors help to sustain it from the stem cell stage of satellite cells, MyoD and Myf-5 act as myogenic determinants in the myoblast’s diet, and myogenin and MRF4 are known as regulators of the later differentiation and muscle fiber formation (Pallari, 2011). Other factors Although some factors that induce differentiation remain unknown, some growth factors and signaling molecules have been shown to regulate the process. Notch signaling is important in animal embryonic development, in that it participates in the cell fate determination (Alberts et al., 2008). Notch has a dual role of myoblasts in that the protein has an inhibitory effect on myoblasts differentiation (Shawber et al., 1996) and simultaneously stimulates their proliferation (Conboy and Rando, 2002). The chemoattractant SDF-1 also has an inhibitory effect on myoblasts differentiation and stimulates their proliferation by activation of the PKCÃŽ ¶ (Ãâ€"demiÃ…Å ¸ et al., 2007). Moreover, differentiation requires the expression and activity of cyclindependent kinase (Cdk) inhibitors, such as p21 and p27, critical for the withdrawal of myoblasts from the cell cycle (Kitzmann and Fernandez, 2001). To fully understand the factors that regulate, activate and inhibit satellite cells and their differentiation requires even much research (Kuang and Rudnicki, 2007; Shi and Garry, 2006.). [1] developmental biology Scott gilbert [2] developmental biology Scott gilbert

5 Important School and Statutory Framework Essay

Thousands of Muslims were becoming followers of Guru Hargobind Sahib Ji. Khawaja Raushan was a very respected Muslim Faqir with thousands of people visiting him to pay him respect and get boons. However Khawaja Raushan’s own mind was restless, he was in search of a true Guru. Once a friend told him about Guru Ji, Khawaja Raushan reached Kiratpur and met Guru JI. When he heard Guru Ji’s he was so impressed that he decided to stay at Kiratpur. He became an admirer of Guru Sahib; he started doing Seva of Guru Ji’s horses. One day Guru Ji went out for riding his horse, Khawaja Raushan ran behind Guru Ji in Bairag. See more: Sleep Deprivation Problem Solution Speech Essay He ran more than a mile after Guru Ji, who then dismounted his horse and embraced Khawaja Raushan with great love. Guru Ji then gave Khawaja Raushan Charan Amrit and Naam as well as spiritual Gian and sent him to Doaba to preach Sikhi.Khawaja Raushan was going from one village to another for preaching Sikhi, one day he met Sayyad Jaani Shah who was wandering in search of bliss. Jaani Shah had met many Sadhus and Faqirs but could not get spiritual Gian from anyone. Khawaja Raushan told him he should go to Kiratpur to achieve your aim. Firstly Jaani Shah did not believe him but when Khawaja Raushan told his story he was determined to go. When he reached Kiratpur and sat in front of the door of Guru Ji’s house and cried loudly â€Å"Janni Ko Jaani Milaa Do (Let Jaani meet his dear one )†.

North American History Essay

In the words of Abraham Lincoln and Stephen A. Douglas, two great rivals in American History, were not only their honor and image, but the principles and ideals that would lead the legislation of a country in the frustrated process of amalgamation and integration. As Abraham Lincoln proposed in his Republican State Convention of 1858 speech, there were two American clashing ideologies in debate, ideologies that could not coexist forever within a â€Å"House Divided†. Moreover, he emphasizes his beliefs when he states: â€Å"Either the opponents of slavery will arrest the further spread of it, and place it where the public mind shall rest in the belief that it is in the course of ultimate extinction, or its advocates will push it forward till it shall become alike lawful in all the States, old as well as new, North as well as South† (Lincoln) By his immediate rhetorical question of â€Å"Have we no tendency to the latter condition?† (Lincoln) it is obvious that he, as a republican and abolitionist, is against the prevailing of the institution of slavery, something that is put into doubt by the accusations made to him by his opponent who adheres himself to prove the lack of congruence in Lincoln’s speeches. This accusation can be tangible to a point, for the speeches were more emphatic towards certain ideas in the north, than in the south and vice versa, but the main principles of Lincoln’s ideas tend to show his point of view as aligned with that of the Abolitionists, in quite a particular way. Taking into account certain confusing ambivalence in Lincoln’s speech, although he proposed equality when he invited Americans to â€Å"†¦unite as one people throughout this land until we shall once more stand up declaring that all men are created equal.† (Douglas, quoting Lincoln), he also encouraged certain division and differentiated whites from blacks when he said â€Å"†¦I am not nor ever have been in favor of bringing about in any way, the social and political equality of the white and black races†¦Ã¢â‚¬  (Douglas, quoting Lincoln), referring to certain ways of life and the position to be held by African Americans in his opinion. A difference in these quotations is evident, but it is taken as a cautious step towards safekeeping his political career, and expressing a certain opinion that supported the abolition of slavery, but not intend to radically change the position of hegemony of the white men. A great conflict can come out of this ideology, b ut it was a great step forward in the emancipation of the black people. Although Abraham Lincoln’s idea of the condition of humans as slaves is left idle for a moment, the fact that he takes it to be an evil that must be stopped is clear when â€Å"†¦we think it is a moral, a social and a political wrong.† (Lincoln) is stated, but the fact that it should be dealt with â€Å"†¦as with any other wrong, in so far as we can prevent its growing any larger, and so deal with it that in the run of time there may be some promise of an end to it.† (Lincoln) also makes clear that although intervention in the south is not within his policy, it is his intention to stop its growth and contain it within its boundaries until, as stated in another occasion, it would perish. In our opinion, Lincoln’s plan is very difficult thing to attain, for how could one oppress an institution such as slavery, and promote openly pro north policies, supposedly leaving the south to their ideals until the times and abolitionist movement created such a situation where the institution would collapse by itself? This is what Judge Douglas questions the most, making Lincoln seem as incoherent. To a point he was, but the great tension created between both ideologies had separated the country to a point in which Lincoln had to have great care. The Missouri compromise dividing free and slave states in the latitude parallel 36à ¯Ã‚ ¿Ã‚ ½30†² and the later Kansas Nebraska act only temporally detained the and smoothed the tension that existed in the fight for power and representation between North and South, abolitionists and slave supporters. The Dred Scott versus Stanford case only augmented the tension, making the questions of its constitutionality and rightfulness be debated across the nation. While Douglas’s position tends to favor a patched agreement between south and north, one of â€Å"mutual non intervention† (Douglas) that could lead to further separation as we think, Lincoln’s policy, ambiguous as it might be in certain respects, was very clear in one thing: the separation that up to now had existed dividing the country in two could not continue much further. For him, apart of his abolitionist principles, above all was the union of the Federation, he could not permit each part of the nation to take its own course, something that would change the fate of the United States forever. A pivotal period would come out of his later governance that would lean the countries future northwards, until again unity would rise much later. This intention would not be clear since the beginning of Lincoln’s political career, despite his clear ideals regarding slavery. This is obvious in the difference in his â€Å"House Divided† speech, and his Sixth Joint Debate with Douglas, at Quincy. Even in the different speeches mentioned in the second paper, where the words of this leader can be confusing, there is certain continuity in his thought. The Dred Scott case is very important in both of his situations. In the first one he addresses it as if the slave policies were â€Å"tending† (Lincoln) the nation towards them, in the second one he is reassuring the possibilities this interpretation of the constitution of the United States by the Supreme Court opens, such as â€Å"†¦slavery would be established in all the States as well as in the Territories.† (Lincoln) Within these lines, the point of view of Abraham Lincoln and Stephen A. Douglas is made clear. While the first clearly wants to avoid the spreading of slavery, for the moment being, and eventually eradicate it, Douglas, claiming to have a â€Å"care-not† (Lincoln) policy as stated by Lincoln, endorsed the popular sovereignty doctrine. It is Douglas who previously had proposed the Kansas-Nebraska Act, giving power of decision on the free or slave state issue to the inhabitants of the area, not based on the moral and ethical consequences of this, for he was not concerned with these but more so in â€Å"expansion of settlement and commerce† (Divine, Robert, et al. 271). Both politicians have a diverging speech and line of thought, for Abraham Lincoln concentrates more on repudiating the idea of slavery itself, while Douglas is not focuses on this, but rather on another scheme: one which was more pleasing to draw support from both sides, one that was in the middle of supporting and fighting slavery, one which proved a failure as the book just cited comments, for the two currents of thought, the one supporting and the other rejecting slavery are very difficult to unite as a whole. It can be said that all these ideological questions surge between attacks and allegations against each other. The first â€Å"House Divided† speech is more of a uplifting, but challenging and persuasive speech in which Lincoln confronts the problems of the nation, the second speech, or debate between Lincoln and Douglas is more of a defensive and offensive one in which one politician is going to enhance his reputation, beneficial for a further political career and the other is going to worsen it. This is obvious for most of the speech is consumed in correcting fallacies said by the other or accusations of perjuries. Between lines is when the true nature of the politicians though reveals itself with clarity. â€Å"It is precisely no other than the putting of that most unphilosophical proposition, that two bodies can occupy the same space at the same time.† (Lincoln) is the quotation that convinces the readers of the Lincoln-Douglas debate of America’s strain, the point of view of slavery cannot coexist with abolitionism, not even Douglas’s view of giving the choice to the inhabitants of the place is viable. Of course Lincoln has to be careful with such a topic, and he is, sounding flexible when he states that â€Å"Judge Douglas understands the Constitution according to the Dred Scott decision, and he is bound to support it as he understands it. I understand it another way, and therefore I am bound to support it in the way in which I understand it.† (Lincoln), yet firm in his convictions. In conclusion, it can be said that the rivalry of these men and ideals they stand for are representative of a nation, a nation that is divided by many issues, of which slavery is a crucial one in understanding the different semi spheres that were being created within what was supposed to be a federation. Although the role of it as such had not yet been clearly defined, it was up for Abraham Lincoln, after he was elected leader, to define with these speeches and hints were making more evident. The American Civil War was just a step away, and Lincoln, Douglas and the slave owners could not agree on the topic that could free a large proportion of the population, the African slaves. Based on what we see, it is clear that what was to come would shape America into what it is now, a united, slave free nation. Yet these are the roots of what is taken for granted today. This antagonism fueled some of the fiercest and defining battles fought in U.S. continental grounds. Bibliography * Divine, Robert A., Breen, T.H., Fredrickson, George M., Williams, R. Hal, Gross, Ariela J., Brands, H.W. and Roberts, Randy † America: Past and Present† United States, Pearson Longman, 2005. * Lincoln, Abraham â€Å"House Divided Address† Republican State Convention, Springfield, Illinois, June 17th, 1858. * Lincoln, Abraham and Douglas, Stephen A. â€Å"Sixth Joint Debate† Lincoln-Douglas Debates, Quincy, Illinois, October 13th, 1858.

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